R Factor-Mediated and Chromosomal Resistance to Ampicillin in Escherichia coli

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R factor-mediated and chromosomal resistance to ampicillin in Escherichia coli.

Sixty-four ampicillin-resistant strains of Escherichia coli were studied. Six characters were examined: (i) resistance to ampicillin, cephalothin, and carbenicillin, (ii) synergy between ampicillin and cloxacillin, (iii) level of beta-lactamase activity after osmotic shock, (iv) transferability of ampicillin resistance, (v) immunological characterization of the enzyme, and (vi) determination of...

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Conjugal fertility associated with resistance factor R in Escherichia coli.

Sugino, Yoshinobu (Osaka University, Osaka, Japan) and Yukinori Hirota. Conjugal fertility associated with resistance factor R in Escherichia coli. J. Bacteriol. 84:902-910. 1962.-The introduction of the contagious drug-resistance factor, R, into an F(-) strain of Escherichia coli allows the R(+)F(-) strain to mate with F(-)R(-) strains. The chromosome fragment transferred from the R(+) cell is...

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Anaerobic R factor transfer in Escherichia coli.

R factor mediated transfer of antibiotic resistance between Enterobacteriaceae has been reported to occur in the mammalian gastrointestinal tract (Kasuya, I 964; Reed, Sieckmann & Georgi, 1969; Guinie, 1970; Wiedemann, Knothe & Doll, 1970) where conditions are mainly anaerobic as demonstrated by the abundance of obligate anaerobic bacteria such as Bacteroides species (Moore, Cat0 & Holdeman, 19...

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Ampicillin susceptibility and ampicillin-induced killing rate of Escherichia coli.

The killing rate induced by ampicillin was determined in 20 strains of Escherichia coli. The apparent generation rate constant for each E. coli showed a characteristic concentration-dependent course. This course can be mathematically described and is determined by four parameters. Three of these parameters determine the speed of the process, and the fourth parameter determines a minimal concent...

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ampC beta-lactamase hyperproduction in Escherichia coli: natural ampicillin resistance generated by horizontal chromosomal DNA transfer from Shigella.

Six ampicillin-resistant clinical isolates of Escherichia coli that hyperproduced the chromosomal ampC beta-lactamase were studied. By DNA sequence analysis, we found that five of them were identical over an entire 449-base-pair sequence and carried a novel strong ampC promoter [Olsson, O., Bergström, S. & Normark, S. (1982) EMBO J. 1, 1411-1416]. Except for one base pair this sequence was iden...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1974

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.5.2.186